Andrea Scrima, Dr. rer. nat.

Position: Postdoc

Room no.: CP-02-114

Telephone no.: +49 231 755 7052

Member of the group since: 10/2023

University degree: Dr. rer. nat. Chemistry, Max Planck Institute of Molecular Physiology/Ruhr-Universität Bochum

 

Expertise: Protein Biochemistry, Molecular Biology, X-Ray Crystallography, Biophysics

 

 

Earlier positions:

  • Postdoctoral position at the Martin-Luther-Universität Halle-Wittenberg, Germany. CORONAmem, Prof. Dr. Milton T. Stubbs, Prof. Dr. Kirsten Bacia, Jun.-Prof. Panagiotis Kastritis
  • Scientist at the Martin-Luther-Universität Halle-Wittenberg, Germany, Group of Prof. Dr. K. Bacia
  • Postdoctoral position at the Helmholtz-Centre for Infection Research, Braunschweig, Germany, Department Structure and Function of Proteins, Group of Prof. Dr. W. Blankenfeldt
  • Head of the Young Investigator Group “Structural Biology of Autophagy” at the Helmholtz-Centre for Infection Research, Braunschweig, Germany, Department Structure and Function of Proteins
  • Postdoctoral position at the Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland, Department Growth Control, Structural Tumour Biology, Group of Dr. N. Thomä
  • Ph. D./Postdoctoral position at the Max-Planck-Institute of Molecular Physiology, Dortmund, Germany, Department Structural Biology, Group of Prof. Dr. A. Wittinghofer

 

Publications:

  1. Schütz, C., Hodzic, A., Hamed, M., Abdelsamie, A.S., Kany, A.M., Bauer, M., Röhrig, T., Schmelz, S., Scrima, A., Blankenfeldt, W., Empting, M. (2021) Divergent synthesis and biological evaluation of 2-(trifluoromethyl)pyridines as virulence-attenuating inverse agonists targeting PqsR. Eur J Med Chem. Dec 15;226:113797.
  2. Schütz, C., Ho, D.K., Hamed, M.M., Abdelsamie, A.S., Röhrig, T., Herr, C., Kany, A.M., Rox, K., Schmelz, S., Siebenbürger, L., Wirth, M., Börger, C., Yahiaoui, S., Bals, R., Scrima, A., Blankenfeldt, W., Horstmann, J.C., Christmann, R., Murgia, X., Koch, M., Berwanger, A., Loretz, B., Hirsch, A.K.H., Hartmann, R.W., Lehr, C.M., Empting, M. (2021) A New PqsR Inverse Agonist Potentiates Tobramycin Efficacy to Eradicate Pseudomonas aeruginosa Biofilms. Adv Sci (Weinh). Jun;8(12):e2004369.
  3. Auerswald, J., Ebenhan, J., Schwieger, C., Scrima, A., Meister, A., Bacia, K. (2021) Measuring protein insertion areas in lipid monolayers by fluorescence correlation spectroscopy. Biophys J. Apr 20;120(8):1333-1342.
  4. Knittel, V., Sadana, P., Seekircher, S., Stolle, A.S., Körner, B., Volk, M., Jeffries, C.M., Svergun, D.I., Heroven, A.K., Scrima, A., Dersch, P. (2020) RovC - a novel type of hexameric transcriptional activator promoting type VI secretion gene expression. PLoS Pathog. Sep 23;16(9):e1008552.
  5. Juettner, N.E., Schmelz, S., Anderl, A., Colin, F., Classen, M., Pfeifer, F., Scrima, A., Fuchsbauer, H.L. (2020) The N-terminal peptide of the transglutaminase-activating metalloprotease inhibitor from Streptomyces mobaraensis accommodates both inhibition and glutamine cross-linking sites. FEBS J. Feb;287(4):708-720.
  6. Elfiky, A., Bonifacius, A., Pezoldt, J., Pasztoi, M., Chaoprasid, P., Sadana, P., El-Sherbeeny, N., Hagras, M., Scrima, A., Dersch, P., Huehn, J. (2018) Yersinia Pseudotuberculosis Modulates Regulatory T Cell Stability via Injection of Yersinia Outer Proteins in a Type III Secretion System-Dependent Manner. Eur J Microbiol Immunol (Bp). Nov 28;8(4):101-106.
  7. Juettner, N.E.*, Schmelz, S.*, Kraemer, A., Knapp, S., Becker, B., Kolmar, H., Scrima, A., Fuchsbauer, H.L.§ (2018) Structure of a glutamine donor mimicking inhibitory peptide shaped by the catalytic cleft of microbial transglutaminase. FEBS J. Dec;285(24):4684-4694.
  8. Fiebig, D., Storka, J., Roeder, M., Meyners, C., Schmelz, S., Blankenfeldt, W., Scrima ,A., Kolmar, H., Fuchsbauer, H.L. (2018) Destructive twisting of neutral metalloproteases: the catalysis mechanism of the Dispase autolysis-inducing protein from Streptomyces mobaraensis DSM 40487. FEBS J. Nov;285(22):4246-4264.
  9. Rebets, Y.*, Schmelz S.*, Gromyko O., Tistechok S., Petzke L., Scrima A.§, Luzhetskyy A.§ (2018) Design, development and application of whole-cell based antibiotic-specific biosensor. Metabolic Engineering. 2018 May;47:263-270.
  10. Sadana P., Geyer R., Pezoldt J., Helmsing S., Huehn J., Hust M., Dersch P., Scrima A. (2018) The invasin D protein from Yersinia pseudotuberculosis selectively binds the Fab region of host antibodies and affects colonization of the intestine. J Biol Chem. 2018 Jun 1;293(22):8672-8690.
  11. Juettner N.E., Schmelz S., Bogen J.P., Happel D., Fessner W.D., Pfeifer F., Fuchsbauer H.L., Scrima A. (2018) Illuminating structure and acyl donor sites of a physiological transglutaminase substrate from Streptomyces mobaraensis. Protein Sci. 2018 May;27(5):910-922
  12. Archna A., Scrima A. (2017) Identification, biochemical characterization and crystallization of the central region of human ATG16L1. Acta Crystallogr F Struct Biol Commun. 73:560-567.
  13. Chaudhry M.Z., Kasmapour B., Plaza-Sirvent C., Bajagic M., Casalegno Garduño R., Borkner L., Lenac Roviš T., Scrima A., Jonjic S., Schmitz I., Cicin-Sain L. (2017) UL36 Rescues Apoptosis Inhibition and In vivo Replication of a Chimeric MCMV Lacking the M36 Gene. Front Cell Infect Microbiol. 7:312.
  14. Bajagic M., Archna A., Büsing P., Scrima A. (2017) Structure of the WD40-domain of human ATG16L1. Protein Science. 26(9):1828-1837.
  15. Sadana P., Mönnich M., Unverzagt C., Scrima A. (2017) Structure of the pseudotuberculosis adhesin InvasinE. Protein Science. 26(6):1182-1195.
  16. Leupold S., Büsing P., Mas P., Hart D., Scrima A. (2017) Structural insights into the architecture of the Shigella flexneri virulence factor IcsA/VirG and motifs involved in polar distribution and secretion. Journal of Structural Biology. 198(1):19-27.
  17. Bock T., Volz C., Hering V., Scrima A., Müller R., Blankenfeldt W. (2016) The AibR-isovaleryl coenzyme A regulator and its DNA binding site - a model for the regulation of alternative de novo isovaleryl coenzyme A biosynthesis in Myxococcus xanthus. Nucleic Acids Research. 45(4):2166-2178.
  18. Fiebig D.*, Schmelz S.*, Zindel S., Ehret V., Beck J., Ebenig A., Ehret M., Fröls S., Pfeifer F., Kolmar H., Fuchsbauer H.L.§, Scrima A.§ (2016) Structure of the dispase autolysis inducing protein from Streptomyces mobaraensis and glutamine cross-linking sites for the attachment of transglutaminase. J Biol Chem. 291(39):20417-26.
  19. Kühne C., Singer H., Grabisch E., Scrima, A., Erhardt M. (2016) RflM mediates specificity of the RcsCDB phosphorelay system for transcriptional repression of flagellar synthesis in Salmonella enterica. Molecular Microbiology. Sep;101(5):841-55.
  20. Bleckmann M., Schmelz S., Schinkowski C., Scrima A., van den Heuvel J. (2016) Fast plasmid based expression screen in insect cells using Split-GFP. Biotechnology and Bioengineering. Sep;113(9):1975-83.
  21. Siegmund V., Schmelz S., Dickgiesser S., Beck J., Ebenig A., Fittler H., Frauendorf H., Piater B., Betz U.A., Avrutina O., Scrima, Fuchsbauer H.L., Kolmar H. (2015) Locked by Design: A Conformationally Constrained Transglutaminase Tag Enables Efficient Site-Specific Conjugation. Angew Chem Int Ed Engl. 54(45):13420-4.
  22. Shi T., Bunker R.D., Mattarocci S., Ribeyre C., Faty M., Gut H., Scrima A., Rass U., Rubin S.M., Shore D., Thomä N.H. (2013) Rif1 and Rif2 shape telomere function and architecture through multivalent Rap1 interactions. Cell 153(6):1340-53.
  23. Hofmeyer T., Schmelz S., Degiacomi M.T., Dal Peraro M., Daneschdar M., Scrima A., van den Heuvel J., Heinz D.W., Kolmar H. (2012) Arranged Sevenfold: Structural Insights into the C-Terminal Oligomerization Domain of Human C4b-Binding Protein. J Mol Biol. 12, 950-953.
  24. Scrima, A., Fischer, E.S., Lingaraju, G.M., Bohm, K., Cavadini, S., and Thomä, N.H. (2011). Detecting UV-lesions in the genome: The modular CRL4 ubiquitin ligase does it best! FEBS Lett 585, 2818-2825.
  25. Fischer E.S.*, Scrima A.*, Böhm K., Matsumoto S., Lingaraju G.M., Faty M., Yasuda T., Cavadini S., Wakasugi M., Hanaoka F., Iwai S., Gut H., Sugasawa K., Thoma N.H. (2011) The molecular basis of CRL4DDB2/CSA ubiquitin ligase architecture, targeting, and activation, Cell 147, 1024 – 1039.
  26. Scrima A., Konícková R., Czyzewski B.K., Kawasaki Y., Jeffrey P.D., Groisman R., Nakatani Y., Iwai S., Pavletich N.P., Thomä N.H. (2008) Structural basis of UV DNA-damage recognition by the DDB1-DDB2 complex. Cell 135(7):1213-1223.
  27. Meyer S., Scrima A., Versées W., Wittinghofer A. (2008) Crystal structures of the conserved tRNA-modifying enzyme GidA: implications for its interaction with MnmE and substrate. J Mol Biol. 380(3):532-547.
  28. Scrima A., Thomas C., Deaconescu D., Wittinghofer A. (2008) The Rap-RapGAP complex: GTP hydrolysis without catalytic glutamine and arginine residues. EMBO J. 27(7):1145-1153.
  29. Thomas C., Fricke , Scrima A., Berken A., Wittinghofer A. (2007) Structural Evidence for a Common Intermediate in Small G Protein-GEF Reactions. Mol Cell. 25(1):141-149.
  30. Gasper R., Scrima A., Wittinghofer A. (2006) Structural insights into HypB, a GTP-binding protein that regulates metal binding. J Biol Chem. 281(37): 27492-27502.
  31. Scrima A., Wittinghofer A. (2006) Dimerisation-dependent GTPase reaction of MnmE: how potassium acts as GTPase-activating element. EMBO J. 25(12):2940-51.
  32. Lammers M., Rose R., Scrima A., Wittinghofer A. (2005) The regulation of mDia1 by autoinhibition and its release by Rho*GTP. EMBO J. 24(23):4176-87.
  33. Scrima A., Vetter I.R., Armengod M.E., Wittinghofer A. (2005) The structure of the TrmE GTP-binding protein and its implications for tRNA modification. EMBO J. 24(1):23-33.

 

* equal contribution, § co-correspondence

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